nf kb ligand rankl (R&D Systems)
Structured Review

Nf Kb Ligand Rankl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nf+kb+ligand+rankl/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc11008737-62-19-22
Average 98 stars, based on 357 article reviews
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1) Product Images from "Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors"
Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
Journal: JBMR Plus
doi: 10.1093/jbmrpl/ziae029
Figure Legend Snippet: Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and RANKL for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.
Techniques Used: Isolation, Staining
Figure Legend Snippet: Mandibular–derived osteoclast precursors proliferate less than femoral–derived osteoclast precursors and do not exhibit an increase in apoptosis activity. Bone marrow cells were flushed from the mandible and femur of 2–month–old C57Bl/6 mice and monocytes were selected. (A) Monocytes were grown in M-CSF for 4 days. CCK-8 activity was measured at 450 nm at plating (day 0) or after 4 days in M-CSF. (B) Osteoclast precursors were grown in M-CSF (day 0) or M-CSF and RANKL for 2 days (day 2). Apoptosis activity was quantified at day 0 or 2 using Promega Caspase-Glo 3/7 Assay. Graphed data represent Caspase-Glo3/7 activity normalized to the average number of nuclei in each culture condition ( N ≥ 3). Samples were compared using Student’s t -test.
Techniques Used: Derivative Assay, Activity Assay, CCK-8 Assay, Caspase-Glo Assay
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Isolation:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of Staining:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of Derivative Assay:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of Activity Assay:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of CCK-8 Assay:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of Caspase-Glo Assay:Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of |
