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nf kb ligand rankl  (R&D Systems)


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    Structured Review

    R&D Systems nf kb ligand rankl
    Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and <t>RANKL</t> for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.
    Nf Kb Ligand Rankl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf+kb+ligand+rankl/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc11008737-62-19-22
    Average 98 stars, based on 357 article reviews
    nf kb ligand rankl - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors"

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziae029

    Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and RANKL for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.
    Figure Legend Snippet: Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and RANKL for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.

    Techniques Used: Isolation, Staining

    Mandibular–derived osteoclast precursors proliferate less than femoral–derived osteoclast precursors and do not exhibit an increase in apoptosis activity. Bone marrow cells were flushed from the mandible and femur of 2–month–old C57Bl/6 mice and monocytes were selected. (A) Monocytes were grown in M-CSF for 4 days. CCK-8 activity was measured at 450 nm at plating (day 0) or after 4 days in M-CSF. (B) Osteoclast precursors were grown in M-CSF (day 0) or M-CSF and RANKL for 2 days (day 2). Apoptosis activity was quantified at day 0 or 2 using Promega Caspase-Glo 3/7 Assay. Graphed data represent Caspase-Glo3/7 activity normalized to the average number of nuclei in each culture condition ( N ≥ 3). Samples were compared using Student’s t -test.
    Figure Legend Snippet: Mandibular–derived osteoclast precursors proliferate less than femoral–derived osteoclast precursors and do not exhibit an increase in apoptosis activity. Bone marrow cells were flushed from the mandible and femur of 2–month–old C57Bl/6 mice and monocytes were selected. (A) Monocytes were grown in M-CSF for 4 days. CCK-8 activity was measured at 450 nm at plating (day 0) or after 4 days in M-CSF. (B) Osteoclast precursors were grown in M-CSF (day 0) or M-CSF and RANKL for 2 days (day 2). Apoptosis activity was quantified at day 0 or 2 using Promega Caspase-Glo 3/7 Assay. Graphed data represent Caspase-Glo3/7 activity normalized to the average number of nuclei in each culture condition ( N ≥ 3). Samples were compared using Student’s t -test.

    Techniques Used: Derivative Assay, Activity Assay, CCK-8 Assay, Caspase-Glo Assay

    Related Articles

    Isolation:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Staining:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Derivative Assay:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Activity Assay:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    CCK-8 Assay:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Caspase-Glo Assay:

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors
    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.



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    Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and <t>RANKL</t> for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.
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    Image Search Results


    Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and RANKL for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.

    Journal: JBMR Plus

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors

    doi: 10.1093/jbmrpl/ziae029

    Figure Lengend Snippet: Monocytes isolated from mandible bone marrow have enhanced osteoclast differentiation. Bone marrow cells were flushed from mandibular and femoral bone and monocytes were selected for osteoclast cultures from 2–month–old mice. (A) Schematic illustrating procedure for selecting monocytes from bone marrow for osteoclast cultures; (B) representative TRAP–stained images of monocytes differentiated into osteoclasts in the presence of M-CSF and RANKL for 4 days. (C) Quantification of the number of TRAP positive multinuclear cells ( N ≥ 3). (D) Quantification of size of TRAP positive multinuclear cells. Scale bar is 10 mm.

    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Techniques: Isolation, Staining

    Mandibular–derived osteoclast precursors proliferate less than femoral–derived osteoclast precursors and do not exhibit an increase in apoptosis activity. Bone marrow cells were flushed from the mandible and femur of 2–month–old C57Bl/6 mice and monocytes were selected. (A) Monocytes were grown in M-CSF for 4 days. CCK-8 activity was measured at 450 nm at plating (day 0) or after 4 days in M-CSF. (B) Osteoclast precursors were grown in M-CSF (day 0) or M-CSF and RANKL for 2 days (day 2). Apoptosis activity was quantified at day 0 or 2 using Promega Caspase-Glo 3/7 Assay. Graphed data represent Caspase-Glo3/7 activity normalized to the average number of nuclei in each culture condition ( N ≥ 3). Samples were compared using Student’s t -test.

    Journal: JBMR Plus

    Article Title: Mouse mandibular–derived osteoclast progenitors have differences in intrinsic properties compared with femoral–derived progenitors

    doi: 10.1093/jbmrpl/ziae029

    Figure Lengend Snippet: Mandibular–derived osteoclast precursors proliferate less than femoral–derived osteoclast precursors and do not exhibit an increase in apoptosis activity. Bone marrow cells were flushed from the mandible and femur of 2–month–old C57Bl/6 mice and monocytes were selected. (A) Monocytes were grown in M-CSF for 4 days. CCK-8 activity was measured at 450 nm at plating (day 0) or after 4 days in M-CSF. (B) Osteoclast precursors were grown in M-CSF (day 0) or M-CSF and RANKL for 2 days (day 2). Apoptosis activity was quantified at day 0 or 2 using Promega Caspase-Glo 3/7 Assay. Graphed data represent Caspase-Glo3/7 activity normalized to the average number of nuclei in each culture condition ( N ≥ 3). Samples were compared using Student’s t -test.

    Article Snippet: Two days later, cells were refed with 1.5% CMG 14-12 culture supernatant and 5 ng/mL of receptor activator of NF-kB ligand (RANKL) (R and D Systems, catalog #462-TEC-010) to stimulate osteoclast differentiation.

    Techniques: Derivative Assay, Activity Assay, CCK-8 Assay, Caspase-Glo Assay

    Expression of osteoclastic differentiation markers. ( A ) The mean expression of osteoclast RANKL in freeze-dried bovine bone xenograft (FDBX) and composite groups decreases from 2 through 8 weeks of healing as opposed to that in the control groups which show gradual increase with time. The mean expression of osteoclastic RANKL in the control groups is significantly higher ( p < 0.05) than the FDBX and composite group at 4 and 8 weeks of healing. ( B ) The mean osteoblast expression of osteoprotegerin (OPG) protein increases gradually through the observation periods, no significant difference is observed in OPG expressions between the composite and FDBX groups ( p > 0.05) during the same periods of healing. ( C ) The OPG/RANKL ratio in the composite groups is much higher, > twofold those in the FDBX group specifically at 4 and 8 weeks indicating more osteoblastic activities in the composite group.

    Journal: European Journal of Dentistry

    Article Title: Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

    doi: 10.1055/s-0041-1736291

    Figure Lengend Snippet: Expression of osteoclastic differentiation markers. ( A ) The mean expression of osteoclast RANKL in freeze-dried bovine bone xenograft (FDBX) and composite groups decreases from 2 through 8 weeks of healing as opposed to that in the control groups which show gradual increase with time. The mean expression of osteoclastic RANKL in the control groups is significantly higher ( p < 0.05) than the FDBX and composite group at 4 and 8 weeks of healing. ( B ) The mean osteoblast expression of osteoprotegerin (OPG) protein increases gradually through the observation periods, no significant difference is observed in OPG expressions between the composite and FDBX groups ( p > 0.05) during the same periods of healing. ( C ) The OPG/RANKL ratio in the composite groups is much higher, > twofold those in the FDBX group specifically at 4 and 8 weeks indicating more osteoblastic activities in the composite group.

    Article Snippet: Samples were stained for 30 minutes with mouse monoclonal anti-rabbit Runx2, receptor activator of NF-kB ligand (RANKL), and osteoprotegerin (OPG) antibodies (Santa Cruz Biotechnology Inc., USA), mouse monoclonal anti-rabbit osteocalcin, collagen-I, and alkaline phosphatase (ALP) (Novus Biological, USA).

    Techniques: Expressing, Control